H2AFY antibody

Catalog No. ABIN7605472

The Rabbit Monoclonal anti-H2AFY antibody is suitable to detect H2AFY in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF and ICC.

Quick Overview for H2AFY antibody (ABIN7605472)

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFY antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: ADDI-8

Product Details anti-H2AFY Antibody

Purpose: Anti-macroH2A.1 H2AFY Monoclonal Antibody

Characteristics: Anti-macroH2A.1 H2AFY Monoclonal Antibody (ABIN7605472). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human macroH2A.1 Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for H2AFY

Target: H2AFY (H2A Histone Family, Member Y (H2AFY))

Alternative Name: H2AFY

Background:

Synonyms: Glycine--tRNA ligase,3.6.1.17 ,6.1.1.14 ,Diadenosine tetraphosphate synthetase,AP-4-A synthetase,Glycyl-tRNA synthetase,GlyRS,GARS,

Tissue Specificity: Widely expressed, including brain and spinal cord. .

Molecular Weight: 38 kDa

UniProt: O75367