NCK1 antibody

Catalog No. ABIN7605666

This Rabbit Monoclonal antibody specifically detects NCK1 in WB, IHC, IF, IP, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NCK1 antibody (ABIN7605666)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: AFEG-14

Product Details anti-NCK1 Antibody

Purpose: Anti-Nck NCK1 Monoclonal Antibody

Characteristics: Anti-Nck NCK1 Monoclonal Antibody (ABIN7605666). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Nck Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background:

Synonyms: Oligodendrocyte transcription factor 2,Oligo2,Class B basic helix-loop-helix protein 1,bHLHb1,Class E basic helix-loop-helix protein 19,bHLHe19,Protein kinase C-binding protein 2,Protein kinase C-binding protein RACK17,OLIG2,BHLHB1, BHLHE19, PRKCBP2, RACK17,

Tissue Specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. .

Molecular Weight: 16 kDa

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals