PHD1 antibody

Catalog No. ABIN7605934

The Rabbit Monoclonal anti-PHD1 antibody has been validated for WB, IHC, FACS, IF and ICC. It is suitable to detect PHD1 in samples from Human, Mouse and Rat.

Quick Overview for PHD1 antibody (ABIN7605934)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: ABD-5

Product Details anti-PHD1 Antibody

Purpose: Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody

Characteristics: Anti-PHD1/prolyl hydroxylase Rabbit Monoclonal Antibody (ABIN7605934). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PHD1/prolyl hydroxylase

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2

Background:

Synonyms: Egl nine homolog 2,1.14.11.29,Estrogen-induced tag 6,HPH-3,Hypoxia-inducible factor prolyl hydroxylase 1,HIF-PH1,HIF-prolyl hydroxylase 1,HPH-1,Prolyl hydroxylase domain-containing protein 1,PHD1,EGLN2,EIT6,

Tissue Specificity: Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. .

Molecular Weight: 35 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis