PKC alpha antibody

Catalog No. ABIN7605963

This Rabbit Monoclonal antibody specifically detects PKC alpha in WB, IHC, IP, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PKC alpha antibody (ABIN7605963)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: CHO-16

Product Details anti-PKC alpha Antibody

Purpose: Anti-PKC alpha PRKCA Rabbit Monoclonal Antibody

Characteristics: Anti-PKC alpha PRKCA Rabbit Monoclonal Antibody (ABIN7605963). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PKC alpha

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA

Background:

Synonyms: Protein kinase C alpha type,PKC-A,PKC-alpha,2.7.11.13,PRKCA,PKCA, PRKACA,

Tissue Specificity: Ubiquitous. Expressed at high levels in testis.

Molecular Weight: 133 kDa

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling