PDHB antibody

Catalog No. ABIN7606102

The Rabbit Monoclonal anti-PDHB antibody has been validated for WB, IHC, IP, ICC, FACS and IF. It is suitable to detect PDHB in samples from Human, Rat and Mouse.

Quick Overview for PDHB antibody (ABIN7606102)

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: AEAD-16

Product Details anti-PDHB Antibody

Purpose: Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody

Characteristics: Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody (ABIN7606102). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) .

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: PDHB

Background:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 5,MAP kinase kinase 5,MAPKK 5,2.7.12.2,MAPK/ERK kinase 5,MEK 5,MAP2K5,MEK5, MKK5, PRKMK5,

Tissue Specificity: Expressed in many adult tissues. Abundant in heart and skeletal muscle.

Molecular Weight: 85 kDa

UniProt: P11177

Pathways: Warburg Effect