SIRPA antibody

Catalog No. ABIN7606294

The Rabbit Monoclonal anti-SIRPA antibody has been validated for WB and FACS. It is suitable to detect SIRPA in samples from Human, Rat and Mouse.

Quick Overview for SIRPA antibody (ABIN7606294)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 19S17

Product Details anti-SIRPA Antibody

Purpose: Anti-SIRP alpha Rabbit Monoclonal Antibody

Characteristics: Anti-SIRP alpha Rabbit Monoclonal Antibody (ABIN7606294). Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human SIRP alpha

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: SIRPA

Background:

Synonyms: Caspase-14,CASP-14,3.4.22.-,Caspase-14 subunit p17, mature form,Caspase-14 subunit p10, mature form,Caspase-14 subunit p20, intermediate form,Caspase-14 subunit p8, intermediate form,CASP14,

Tissue Specificity: Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed:11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846). .

Molecular Weight: 85 kDa

UniProt: P78324