SIRT2 antibody

Catalog No. ABIN7606296

This Rabbit Monoclonal antibody specifically detects SIRT2 in WB, IP, IF, ICC and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for SIRT2 antibody (ABIN7606296)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: EBA-19

Product Details anti-SIRT2 Antibody

Purpose: Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody

Characteristics: Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody (ABIN7606296). Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human SIRT2

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:30

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2

Background:

Synonyms: NAD-dependent protein deacetylase sirtuin-2,3.5.1.-,Regulatory protein SIR2 homolog 2,SIR2-like protein 2,SIRT2,SIR2L, SIR2L2,

Tissue Specificity: Isoform 1 is expressed in heart, liver and skeletal muscle, weakly expressed in the cortex. Isoform 2 is strongly expressed in the cortex, weakly expressed in heart and liver. Weakly expressed in several malignancies including breast, liver, brain, kidney and prostate cancers compared to normal tissues. Weakly expressed in glioma cell lines compared to normal brain tissues (at protein level). Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. .

Molecular Weight: 160-180 kDa