DIABLO antibody

Catalog No. ABIN7606315

The Rabbit Monoclonal anti-DIABLO antibody has been validated for WB, IHC, IF, IP, ICC and FACS. It is suitable to detect DIABLO in samples from Human, Mouse and Rat.

Quick Overview for DIABLO antibody (ABIN7606315)

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: FGG-4

Product Details anti-DIABLO Antibody

Purpose: Anti-Smac/Diablo Rabbit Monoclonal Antibody

Characteristics: Anti-Smac/Diablo Rabbit Monoclonal Antibody (ABIN7606315). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Smac/Diablo

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: DIABLO

Background:

Synonyms: Diablo homolog, mitochondrial, IAP-binding protein with low pI,Second mitochondria-derived activator of caspase,Smac,DIABLO,SMAC,

Tissue Specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. .

Molecular Weight: 54 kDa

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity