DIABLO antibody

Catalog No. ABIN462354

The Mouse Monoclonal anti-DIABLO antibody (Clone 79-1-83) (ABIN462354) specifically detects DIABLO in WB, IHC and IHC (p). The antibody is reactive with Human and Monkey samples.

Quick Overview for DIABLO antibody (ABIN462354)

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This DIABLO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 79-1-83

Product Details anti-DIABLO Antibody

Specificity: Human and monkey Smac/DIABLO.

Purification: Protein G purified

Immunogen: Recombinant full length human Smac/DIABLO.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % azide, 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: DIABLO / SMAC

Background: Name/Gene ID: DIABLO
Family: Apoptosis

Synonyms: DIABLO, DFNA64, DIABLO-S, Diablo homolog, mitochondrial, SMAC3, Mitochondrial Smac protein, SMAC

Gene ID: 56616

UniProt: Q9NR28

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity