ube3a antibody

Catalog No. ABIN7606633

The Rabbit Monoclonal anti-ube3a antibody has been validated for WB. It is suitable to detect ube3a in samples from Human, Mouse and Rat.

Quick Overview for ube3a antibody (ABIN7606633)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Western Blotting (WB)

Clone: ADBA-21

Product Details anti-ube3a Antibody

Purpose: Anti-UBE3A Monoclonal Antibody

Characteristics: Anti-UBE3A Monoclonal Antibody (ABIN7606633). Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human UBE3A E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: UBE3A

Background:

Synonyms: Interferon regulatory factor 1,IRF-1,IRF1,

Tissue Specificity: Heart, placenta, skeletal muscle, kidney, lung and pancreas. .

Molecular Weight: 47 kDa

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway