Anti-Beta4-Spectrin Recombinant Chicken Chimeric mAb (N393/76)
Specificity
No off-targets reported for other Beta-spectrins
Cross-Reactivity
Human, Mouse, Rat
Characteristics
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N393/76 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N393/76 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Purification
Purified by affinity chromatography.
Immunogen
Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin (accession number Q9H254) produced recombinantly in E. Coli
SPTBN4
Reactivity: Human
IHC, WB, ICC, IF
Host: Mouse
Monoclonal
S393-2
Atto 390
Application Notes
WB: 1:1000 IHC: 1:250 ICC: 1:500
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
1X PBS, 0.05 % Sodium Azide 7.4
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.