APOA1 antibody (AA 51-150)

Catalog No. ABIN728968

This Rabbit Polyclonal antibody specifically detects APOA1 in WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc) and IF (p). It exhibits reactivity toward Human, Mouse, Rat and Chicken and has been mentioned in 5+ publications.

Quick Overview for APOA1 antibody (AA 51-150) (ABIN728968)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-APOA1 Antibody

Binding Specificity: AA 51-150

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human APOA1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-APOA1 antibody (ABIN728968)

Zhang, Zhao, Yu, Liu, Hao, Zhang, Ge, Min, Shen, Li, Kou, Ma, Li: "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." in: Theriogenology, Vol. 95, pp. 154-162, (2018) (PubMed).

Wu, Wu, Liu, Wang, Na, Wang, Li: "Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers." in: Proteome science, Vol. 14, Issue 1, pp. 9, (2016) (PubMed).

Hibert, Prunier-Mirebeau, Beseme, Chwastyniak, Tamareille, Lamon, Furber, Pinet, Prunier: "Apolipoprotein a-I is a potential mediator of remote ischemic preconditioning." in: PLoS ONE, Vol. 8, Issue 10, pp. e77211, (2013) (PubMed).

Liu, Zhao, Lu, Fan, Wang: "Proteomic study on usnic-acid-induced hepatotoxicity in rats." in: Journal of agricultural and food chemistry, Vol. 60, Issue 29, pp. 7312-7, (2012) (PubMed).

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1

Background:

Synonyms: Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, APOA1

Background: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Gene ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism