Recombinant PD-L1 antibody

Catalog No. ABIN7630043

The Mouse Monoclonal anti-PD-L1 antibody has been validated for WB, FACS, IHC, IP, IF and ICC. It is suitable to detect PD-L1 in samples from Human.

Quick Overview for Recombinant PD-L1 antibody (ABIN7630043)

Target: PD-L1 (CD274 (PD-L1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: E286-C1

Product Details anti-PD-L1 Antibody

Purpose: Recombinant Antibody to Programmed Cell Death Protein 1 Ligand 1 (PDL1)

Specificity: The antibody is a mouse monoclonal antibody raised against PDL1. It has been selected for its ability to recognize PDL1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL, Immunohistochemistry: 5-20 μg/mL, Immunocytochemistry: 5-20 μg/mL, Flow cytometry:0.1 μg/mL, Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: Programmed Cell Death Protein 1 Ligand 1

Background: CD274, PDCD1LG1, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1-LG1, Programed Death Ligand 1

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints