Recombinant CD8 alpha antibody

Catalog No. ABIN7630057

This Mouse Monoclonal antibody specifically detects CD8 alpha in FACS, IHC, WB, IP, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD8 alpha antibody (ABIN7630057)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CD8 alpha Antibody

Purpose: Recombinant Antibody to Cluster Of Differentiation 8a (CD8a)

Specificity: The antibody is a mouse monoclonal antibody raised against CD8a. It has been selected for its ability to recognize CD8a in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL, Immunohistochemistry: 5-20 μg/mL, Immunocytochemistry: 5-20 μg/mL, Flow cytometry:10 μg/mL, Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: Cluster Of Differentiation 8a

Background: P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

UniProt: P01732

Pathways: TCR Signaling