Recombinant CD73 antibody

Catalog No. ABIN7630169

The Mouse Monoclonal anti-CD73 antibody has been validated for WB, IHC, FACS, IF, ICC and IP. It is suitable to detect CD73 in samples from Human.

Quick Overview for Recombinant CD73 antibody (ABIN7630169)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-CD73 Antibody

Purpose: Recombinant Antibody to Cluster Of Differentiation 73 (CD73)

Specificity: The antibody is a mouse monoclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Isotype: IgG2b kappa

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL, Immunohistochemistry: 5-20 μg/mL, Immunocytochemistry: 5-20 μg/mL, Flow cytometry:5 μg/mL, Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: Cluster Of Differentiation 73

Background: NT5E, NT5-E, E5NT, E5-NT, NTE, EN, ENT, 5'-Nucleotidase, Ecto

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process