14-3-3 zeta antibody (Biotin)

Catalog No. ABIN7630175

The Rabbit Polyclonal anti-14-3-3 zeta antibody has been validated for WB, IHC and ICC. It is suitable to detect 14-3-3 zeta in samples from Human.

Quick Overview for 14-3-3 zeta antibody (Biotin) (ABIN7630175)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-14-3-3 zeta Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

Specificity: The antibody is a rabbit polyclonal antibody raised against YWHAz. It has been selected for its ability to recognize YWHAz in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta

Background: KCIP-1, 14-3-3-zeta, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location