ALT antibody (Biotin)

Catalog No. ABIN7630267

This Rabbit Polyclonal antibody specifically detects ALT in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for ALT antibody (Biotin) (ABIN7630267)

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALT antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-ALT Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Alanine Aminotransferase (ALT)

Specificity: The antibody is a rabbit polyclonal antibody raised against ALT. It has been selected for its ability to recognize ALT in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA207Hu01Polyclonal Antibody to Alanine Aminotransferase (ALT)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: Alanine Aminotransferase

Background: GPT, ALAT, SGPT, AAT1, GPT1, Serum Glutamic Pyruvic Transaminase, Alanine Transaminase, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1

UniProt: P24298