CXCL12 antibody (Biotin)

Catalog No. ABIN7697097

The Biotin-conjugated Rabbit Polyclonal anti-CXCL12 antibody (ABIN7697097) specifically detects CXCL12 in WB, IHC and ICC. The antibody is reactive with Mouse samples.

Quick Overview for CXCL12 antibody (Biotin) (ABIN7697097)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL12 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CXCL12 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Stromal Cell Derived Factor 1 (SDF1)

Specificity: The antibody is a rabbit polyclonal antibody raised against SDF1. It has been selected for its ability to recognize SDF1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA122Mu01Polyclonal Antibody to Stromal Cell Derived Factor 1 (SDF1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: Stromal Cell Derived Factor 1

Background: CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12

UniProt: P40224

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling