CUBN antibody (FITC)

Catalog No. ABIN7701925

This Rabbit Polyclonal antibody specifically detects CUBN in WB, IHC, ICC and IF. It exhibits reactivity toward Mouse.

Quick Overview for CUBN antibody (FITC) (ABIN7701925)

Target: CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CUBN antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-CUBN Antibody (FITC)

Purpose: FITC-Linked Polyclonal Antibody to Cubilin (CUBN)

Specificity: The antibody is a rabbit polyclonal antibody raised against CUBN. It has been selected for its ability to recognize CUBN in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAC411Mu01Polyclonal Antibody to Cubilin (CUBN)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CUBN

Target: CUBN (Cubilin (Intrinsic Factor-Cobalamin Receptor) (CUBN))

Alternative Name: Cubilin

Background: IFCR, MGA1, gp280, Intrinsic Factor Cobalamin Receptor, 460 kDa receptor, Intestinal intrinsic factor receptor, Intrinsic factor-vitamin B12 receptor

UniProt: Q9JLB4

Pathways: Metabolism of Steroid Hormones and Vitamin D, Lipid Metabolism