EPAS1 antibody (Biotin)

Catalog No. ABIN7631251

This Rabbit Polyclonal antibody specifically detects EPAS1 in WB, IHC and ICC. It exhibits reactivity toward Human.

Quick Overview for EPAS1 antibody (Biotin) (ABIN7631251)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPAS1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-EPAS1 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a)

Specificity: The antibody is a rabbit polyclonal antibody raised against HIF2a. It has been selected for its ability to recognize HIF2a in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Alternative Name: Hypoxia Inducible Factor 2 Alpha

Background: EPAS1, HLF, MOP2, PASD2, BHLHE73, Endothelial PAS Domain 1, HIF-1 Alpha-Like Factor, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect