Iba1 antibody (Biotin)

Catalog No. ABIN7632204

This anti-Iba1 antibody is a Mouse Monoclonal antibody detecting Iba1 in WB, IHC and ICC. Suitable for Human.

Quick Overview for Iba1 antibody (Biotin) (ABIN7632204)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-Iba1 Antibody (Biotin)

Purpose: Biotin-Linked Monoclonal Antibody to Ionized Calcium-binding Adapter Molecule 1 (IBA1)

Specificity: The antibody is a mouse monoclonal antibody raised against AIF1. It has been selected for its ability to recognize AIF1 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: Ionized Calcium-binding Adapter Molecule 1

Background: AIF1, IRT1, Daintain, Protein G1, Allograft Inflammatory Factor 1

UniProt: P55008

Pathways: Smooth Muscle Cell Migration