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Podoplanin antibody (Cy3)

The Rabbit Polyclonal anti-Podoplanin antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect Podoplanin in samples from Human.
Catalog No. ABIN7632905

Quick Overview for Podoplanin antibody (Cy3) (ABIN7632905)

Target

See all Podoplanin (PDPN) Antibodies
Podoplanin (PDPN)

Reactivity

  • 91
  • 29
  • 10
  • 3
  • 1
  • 1
  • 1
Human

Host

  • 66
  • 36
  • 6
  • 5
  • 2
  • 2
  • 2
Rabbit

Clonality

  • 64
  • 54
  • 1
Polyclonal

Conjugate

  • 79
  • 11
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Podoplanin antibody is conjugated to Cy3

Application

  • 91
  • 53
  • 36
  • 31
  • 30
  • 23
  • 15
  • 14
  • 8
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Purpose

    Cy3-Linked Polyclonal Antibody to Podoplanin (PDPN)

    Specificity

    The antibody is a rabbit polyclonal antibody raised against PDPN. It has been selected for its ability to recognize PDPN in immunohistochemical staining.

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    PAC719Hu01Polyclonal Antibody to Podoplanin (PDPN)

    Isotype

    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Target

    Podoplanin (PDPN)

    Alternative Name

    Podoplanin

    Background

    GP40, GP36, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, Aggrus, Glycoprotein 36, PA2.26 antigen, T1-alpha, Lung Type I Cell Membrane Associated Glycoprotein

    UniProt

    Q86YL7

    Pathways

    Dicarboxylic Acid Transport
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