CBX5 antibody (pTyr24)

Catalog No. ABIN729943

This Rabbit Polyclonal antibody specifically detects CBX5 in WB, ELISA, IHC (p), IF (p), IF (cc) and IHC (fro). It exhibits reactivity toward Human and Drosophila melanogasterand has been mentioned in 1 publication.

Quick Overview for CBX5 antibody (pTyr24) (ABIN729943)

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivity: Human, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CBX5 Antibody

Binding Specificity: pTyr24

Cross-Reactivity: Human

Cross-Reactivity (Details): Fruit Fly

Predicted Reactivity: Drosophila

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from Drosophila Melanogaster HP1 around the phosphorylation site of Tyr24

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CBX5 antibody (ABIN729943)

Terzuoli, Meini, Cucchi, Catalani, Cialdai, Maggi, Giachetti, Ziche, Donnini: "Antagonism of bradykinin B2 receptor prevents inflammatory responses in human endothelial cells by quenching the NF-kB pathway activation." in: PLoS ONE, Vol. 9, Issue 1, pp. e84358, (2014) (PubMed).

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: HP1

Background:

Synonyms: Heterochromatin protein 1, HP1, Non-histone chromosomal protein C1A9 antigen, CG8409

Background: HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are requiredfor binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.

Gene ID: 34119