S100 Protein (S100) antibody (Biotin)

Catalog No. ABIN7633174

This Rabbit Polyclonal antibody specifically detects in WB, IHC and ICC. It exhibits reactivity toward Rat.

Quick Overview for S100 Protein (S100) antibody (Biotin) (ABIN7633174)

Target: S100 Protein (S100)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details

Purpose: Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein (S100)

Specificity: The antibody is a rabbit polyclonal antibody raised against S100. It has been selected for its ability to recognize S100 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details

Target: S100 Protein (S100)

Alternative Name: S100 Calcium Binding Protein

Background: S100-A1, S100A1, S100-Alpha, S-100 Protein, S-100 protein subunit alpha, S-100 protein alpha chain

UniProt: P35467

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins