Vitamin D3 antibody (Biotin)

Catalog No. ABIN7633757

The Rabbit Polyclonal anti-Vitamin D3 antibody has been validated for ICC, WB and IHC. It is suitable to detect Vitamin D3 in samples from Various Species.

Quick Overview for Vitamin D3 antibody (Biotin) (ABIN7633757)

Target: Vitamin D3

Reactivity: Various Species

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vitamin D3 antibody is conjugated to Biotin

Application: Immunocytochemistry (ICC), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Vitamin D3 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Vitamin D3 (VD3)

Specificity: The antibody is a rabbit polyclonal antibody raised against VD3. It has been selected for its ability to recognize VD3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Vitamin D3

Target: Vitamin D3

Background: Cholecalciferol, Toxiferol, Activated 7-Dehydrocholesterol