CD73 antibody (Biotin)

Catalog No. ABIN7633781

The Rabbit Polyclonal anti-CD73 antibody has been validated for WB, IHC and ICC. It is suitable to detect CD73 in samples from Human.

Quick Overview for CD73 antibody (Biotin) (ABIN7633781)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CD73 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB250Hu01Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: Cluster Of Differentiation 73

Background: NT5E, NT5-E, E5NT, E5-NT, NTE, EN, ENT, 5'-Nucleotidase, Ecto

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process