CD73 antibody (Biotin)

Catalog No. ABIN7633789

This Rabbit Polyclonal antibody specifically detects CD73 in WB, IHC and ICC. It exhibits reactivity toward Rat.

Quick Overview for CD73 antibody (Biotin) (ABIN7633789)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CD73 Antibody (Biotin)

Purpose: Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Specificity: The antibody is a rabbit polyclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAB250Ra01Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: Cluster Of Differentiation 73

Background: NT5E, NT5-E, E5NT, E5-NT, NTE, EN, ENT, 5'-Nucleotidase, Ecto

UniProt: P21588

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process