ARNT antibody

Catalog No. ABIN7635071

The Mouse Monoclonal anti-ARNT antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect ARNT in samples from Human.

Quick Overview for ARNT antibody (ABIN7635071)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: D1

Product Details anti-ARNT Antibody

Purpose: Monoclonal Antibody to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)

Specificity: The antibody is a mouse monoclonal antibody raised against ARNT. It has been selected for its ability to recognize ARNT in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPD470Hu01Recombinant Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: ARNT

Background: HIF-1beta, bHLHe2, Hypoxia Inducible Factor 1 Beta, Class E basic helix-loop-helix protein 2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect