Cyclin B1 antibody

Catalog No. ABIN7637581

This Mouse Monoclonal antibody specifically detects Cyclin B1 in WB, IHC, IP and ICC. It exhibits reactivity toward Human.

Quick Overview for Cyclin B1 antibody (ABIN7637581)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Cyclin B1 Antibody

Purpose: Monoclonal Antibody to Cyclin B (CCNB)

Specificity: The antibody is a mouse monoclonal antibody raised against CCNB. It has been selected for its ability to recognize CCNB in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:500-5000 Immunohistochemistry: 5-20 μg/mL,1:50-200 Immunocytochemistry: 5-20 μg/mL,1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB

Background: CCNB1, CCN-B, G2/Mitotic-Specific Cyclin B1

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase