TOP2 antibody

Catalog No. ABIN7638053

The Rabbit Polyclonal anti-TOP2 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect TOP2 in samples from Mouse.

Quick Overview for TOP2 antibody (ABIN7638053)

Target: TOP2 (DNA Topoisomerase II (TOP2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-TOP2 Antibody

Purpose: Polyclonal Antibody to Topoisomerase II (TOP2)

Specificity: The antibody is a rabbit polyclonal antibody raised against TOP2. It has been selected for its ability to recognize TOP2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Alternative Name: TOP2

Background: DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme

UniProt: Q01320

Pathways: Mitotic G1-G1/S Phases