DDIT3 antibody

Catalog No. ABIN7638059

The Rabbit Polyclonal anti-DDIT3 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect DDIT3 in samples from Rat.

Quick Overview for DDIT3 antibody (ABIN7638059)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DDIT3 Antibody

Purpose: Polyclonal Antibody to DNA Damage Inducible Transcript 3 (DDIT3)

Specificity: The antibody is a rabbit polyclonal antibody raised against DDIT3. It has been selected for its ability to recognize DDIT3 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: DDIT3

Background: CEBPZ, CHOP, CHOP10, GADD153, C/EBP-homologous protein 10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible GADD153

UniProt: Q62857

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis