GCLC antibody
Quick Overview for GCLC antibody (ABIN7639520)
Target
See all GCLC AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC)
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Specificity
- The antibody is a rabbit polyclonal antibody raised against GCLC. It has been selected for its ability to recognize GCLC in immunohistochemical staining and western blotting.
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Isotype
- IgG
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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 528-637) antibody
GCLC Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3H1 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30), (N-Term) antibodyGCLC Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB31687 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Guinea Pig, Horse WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30) antibodyGCLC Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC Reactivity: Human, Mouse, Rat, Pig WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 437-637) antibodyGCLC Reactivity: Human, Mouse, Rat WB, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-252) antibodyGCLC Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibodyGCLC Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (Middle Region) antibodyGCLC Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Guinea Pig, Horse, Saccharomyces cerevisiae WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 6-34), (N-Term) antibodyGCLC Reactivity: Human WB, IF, FACS, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
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Application Notes
- Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.
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Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
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Alternative Name
- GCLC
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Background
- GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS
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UniProt
- P48506
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Pathways
- Cell RedoxHomeostasis
Target
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