CXCR4 antibody (AA 201-294)

Catalog No. ABIN730888

This Rabbit Polyclonal antibody specifically detects CXCR4 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Rabbit and has been mentioned in 16+ publications.

Quick Overview for CXCR4 antibody (AA 201-294) (ABIN730888)

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CXCR4 Antibody

Binding Specificity: AA 201-294

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CXCR4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CXCR4 antibody (ABIN730888)

Sha, Yang, Lv: "MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia-induced cell apoptosis and accelerating angiogenesis." in: Journal of cellular physiology, Vol. 234, Issue 6, pp. 8846-8861, (2019) (PubMed).

Zhuang, Li, Huang, Lei, Yan, Li, Sidhu, Cheng, Yan, Miao, Zhao, Hua: "Reelin regulates male mouse reproductive capacity via the sertoli cells." in: Journal of cellular biochemistry, (2018) (PubMed).

Ochi, Chen, Schulte, Leng, Moeckel, Piecychna, Averdunk, Stoppe, Bucala, Moeckel: "MIF-2/D-DT enhances proximal tubular cell regeneration through SLPI- and ATF4-dependent mechanisms." in: American journal of physiology. Renal physiology, Vol. 313, Issue 3, pp. F767-F780, (2017) (PubMed).

Aerbajinai, Liu, Zhu, Kumkhaek, Chin, Rodgers: "Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin." in: The Journal of biological chemistry, Vol. 291, Issue 16, pp. 8549-64, (2016) (PubMed).

Wang, Chen, Tang, Jiang, Ji: "AMD3100 Attenuates Matrix Metalloprotease-3 and -9 Expressions and Prevents Cartilage Degradation in a Monosodium Iodo-Acetate-Induced Rat Model of Temporomandibular Osteoarthritis." in: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, Vol. 74, Issue 5, pp. 927.e1-927.e13, (2016) (PubMed).

Mori, Matsubara, Matsubara, Uchikura, Hashimoto, Fujioka, Matsumoto: "Stromal Cell-Derived Factor-1? Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 18018-32, (2015) (PubMed).

Mu, Li, Wu, Zheng, Zhai, Li, Song, Wang, Zhu, Li, Hua: "PLZF-Induced Upregulation of CXCR4 Promotes Dairy Goat Male Germline Stem Cell Proliferation by Targeting Mir146a." in: Journal of cellular biochemistry, (2015) (PubMed).

Zhou, Yang, Xin, Zhang, Hu, Zhou, Chen, Chen et al.: "Exendin-4 enhances the migration of adipose-derived stem cells to neonatal rat ventricular cardiomyocyte-derived conditioned medium via the phosphoinositide 3-kinase/Akt-stromal cell-derived ..." in: Molecular medicine reports, (2015) (PubMed).

Zhou, Li, Shi, Xin, Yang, Zhou, Hu, Tian, Wang, Chen: "Effects of Exendin-4 on bone marrow mesenchymal stem cell proliferation, migration and apoptosis in vitro." in: Scientific reports, Vol. 5, pp. 12898, (2015) (PubMed).

Pei, Zeng, Han, Liao, Zhou, Li, Zhang, Liu, Zhang, Liu, Yao, Xu: "Renal interstitial infiltration and tertiary lymphoid organ neogenesis in IgA nephropathy." in: Clinical journal of the American Society of Nephrology : CJASN, Vol. 9, Issue 2, pp. 255-64, (2014) (PubMed).

Wu, Liu, Yan, He, Ye, Cheng, Zhu, Wu, Wang, Kong, Xu, Lobie, Zhu, Wu: "B-cell lymphoma 6 protein stimulates oncogenicity of human breast cancer cells." in: BMC cancer, Vol. 14, pp. 418, (2014) (PubMed).

Li, Shen, Miao, Luan, Sun, Qiu: "Co-expression of uPAR and CXCR4 promotes tumor growth and metastasis in small cell lung cancer." in: International journal of clinical and experimental pathology, Vol. 7, Issue 7, pp. 3771-80, (2014) (PubMed).

Lu, Qi, Bo, Liu, Wang, Li: "Expression of CD26 and CXCR4 in prostate carcinoma and its relationship with clinical parameters." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 18, Issue 8, pp. 647-52, (2013) (PubMed).

Liu, Patzak, Zhang: "CXCR4-overexpressing bone marrow-derived mesenchymal stem cells improve repair of acute kidney injury." in: American journal of physiology. Renal physiology, Vol. 305, Issue 7, pp. F1064-73, (2013) (PubMed).

Zhuo, Jia, Song, Lu, Ding, Wang, Song, Fu, Luo: "The CXCL12-CXCR4 chemokine pathway: a novel axis regulates lymphangiogenesis." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 18, Issue 19, pp. 5387-98, (2012) (PubMed).

Fontana, Vogt, Hohenstein, Vettermann, Doroshenko, Lammer, Yard, Hoeger: "Impact of Steroids on the Inflammatory Response after Ischemic Acute Kidney Injury in Rats." in: Indian journal of nephrology, Vol. 27, Issue 5, pp. 365-371 (PubMed).

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CXCR4

Background:

Synonyms: C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-1 receptor, CD184, CXCR4

Background: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin, leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.

Gene ID: 7852

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events