Sonic Hedgehog antibody (AA 21-120)

Catalog No. ABIN731108

The Rabbit Polyclonal anti-Sonic Hedgehog antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect Sonic Hedgehog in samples from Human, Mouse, Rat and Pig. There are 10+ publications available.

Quick Overview for Sonic Hedgehog antibody (AA 21-120) (ABIN731108)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: AA 21-120

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Shh

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Sonic Hedgehog antibody (ABIN731108)

Fan, Li, Liu, Cong, Zhao, Cao, Zheng: "Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 163, (2018) (PubMed).

Yang, Wu, Feng, Huang, Liu, Liu, Chen: "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 267, (2017) (PubMed).

Song, Du, Tao: "Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway." in: Oncology letters, Vol. 14, Issue 4, pp. 4099-4105, (2017) (PubMed).

Wang, Zhang, Bai, Wang, Du: "Baicalin Attenuates Alcoholic Liver Injury through Modulation of Hepatic Oxidative Stress, Inflammation and Sonic Hedgehog Pathway in Rats." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 3, pp. 1129-40, (2016) (PubMed).

Bai, Lu, Lin, Xu, Hu, Liang, Hong, Chen: "Sonic hedgehog-mediated epithelial-mesenchymal transition in renal tubulointerstitial fibrosis." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1317-27, (2016) (PubMed).

Wu, Zhao, Cheng, Yang, Huang, Meng, Wu, Zhang, Lv, Li: "Melittin induces PTCH1 expression by down-regulating MeCP2 in human hepatocellular carcinoma SMMC-7721 cells." in: Toxicology and applied pharmacology, Vol. 288, Issue 1, pp. 74-83, (2015) (PubMed).

Ma, Li, Li, Yang, Huang, Meng, Zhang, Li: "Geniposide alleviates inflammation by suppressing MeCP2 in mice with carbon tetrachloride-induced acute liver injury and LPS-treated THP-1 cells." in: International immunopharmacology, (2015) (PubMed).

Fan, Wang, Zhao, Liu, Chen, Sun, Zheng: "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 41, (2014) (PubMed).

Weiss, Guo, Shan, Shi, Romano, Sinha, Cantley, Guo: "Brg1 determines urothelial cell fate during ureter development." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 4, pp. 618-26, (2013) (PubMed).

Du, Feng, Wang, Piao, Cui, Chen, Lei, Sun, Liu, Wang, Li, Yang, Sun, Zhao, Jiang, Li, Jiang: "Curcumin suppresses malignant glioma cells growth and induces apoptosis by inhibition of SHH/GLI1 signaling pathway in vitro and vivo." in: CNS neuroscience & therapeutics, Vol. 19, Issue 12, pp. 926-36, (2013) (PubMed).

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Shh

Background:

Synonyms: TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5, Sonic hedgehog protein, SHH, HHG-1

Background: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development