Sonic Hedgehog antibody (AA 21-120) (HRP)

Catalog No. ABIN731117

The Rabbit Polyclonal anti-Sonic Hedgehog antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Sonic Hedgehog in samples from Human, Mouse, Rat and Pig.

Quick Overview for Sonic Hedgehog antibody (AA 21-120) (HRP) (ABIN731117)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Sonic Hedgehog Antibody (HRP)

Binding Specificity: AA 21-120

Cross-Reactivity: Human, Mouse, Pig, Rat

Predicted Reactivity: Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Shh

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Shh

Background:

Synonyms: TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5, Sonic hedgehog protein, SHH, HHG-1

Background: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development