P4HA1 antibody

Catalog No. ABIN7644881

The Rabbit Polyclonal anti-P4HA1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect P4HA1 in samples from Rat.

Quick Overview for P4HA1 antibody (ABIN7644881)

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-P4HA1 Antibody

Purpose: Polyclonal Antibody to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1)

Specificity: The antibody is a rabbit polyclonal antibody raised against P4Ha1. It has been selected for its ability to recognize P4Ha1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPE253Ra01Recombinant Prolyl4Hydroxylase Alpha Polypeptide I (P4Ha1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for P4HA1

Target: P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))

Alternative Name: P4Ha1

Background: Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide I

UniProt: P54001