P4HB antibody

Catalog No. ABIN7644890

The Rabbit Polyclonal anti-P4HB antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect P4HB in samples from Mouse.

Quick Overview for P4HB antibody (ABIN7644890)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-P4HB Antibody

Purpose: Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDI. It has been selected for its ability to recognize PDI in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPB061Mu01Recombinant Protein Disulfide Isomerase (PDI)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: Protein Disulfide Isomerase

Background: P4HB, ERBA2L, PDIA1, PO4DB, PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta, Prolyl 4-Hydroxylase Subunit Beta, Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide

UniProt: P09103

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism