PKC epsilon antibody

Catalog No. ABIN7645049

The Mouse Monoclonal anti-PKC epsilon antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect PKC epsilon in samples from Human.

Quick Overview for PKC epsilon antibody (ABIN7645049)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C9

Product Details anti-PKC epsilon Antibody

Purpose: Monoclonal Antibody to Protein Kinase C Epsilon (PKCe)

Specificity: The antibody is a mouse monoclonal antibody raised against PKCe. It has been selected for its ability to recognize PKCe in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: RPA439Hu01Recombinant Protein Kinase C Epsilon (PKCe)

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PKCe

Background: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling