PKC epsilon antibody (AA 403-672)

Catalog No. ABIN7425860

The Mouse Monoclonal anti-PKC epsilon antibody (Clone C1) (ABIN7425860) specifically detects PKC epsilon in WB, IHC, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for PKC epsilon antibody (AA 403-672) (ABIN7425860)

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: C1

Product Details anti-PKC epsilon Antibody

Binding Specificity: AA 403-672

Purpose: Monoclonal Antibody to Protein Kinase C Epsilon (PKCe)

Specificity: The antibody is a mouse monoclonal antibody raised against PKCe. It has been selected for its ability to recognize PKCe in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Protein Kinase C Epsilon (PKCe) corresdonding to Leu403~Asp672 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,
Immunohistochemistry: 5-20 μg/mL,
Immunofluorescence: 5-20 μg/mL,
Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PKCe

Background: PKC-E, PRKCE, PRKC-E, NPKC-Epsilon

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling