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ATP7A antibody (AA 242-285)

Name: ATP7A antibody (AA 242-285)
SKU: ABIN731513
Price: 384.62 USD
Availability: InStock

There is 1 publication for this product available. The Rabbit Polyclonal anti-ATP7A antibody is suitable to detect ATP7A in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro).

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with ATP7A Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (ATP7A antibody (AA 242-285))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human glioma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with ATP7A Polyclonal Antibody at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (ATP7A antibody (AA 242-285))

IHC (p)

2 images

(1 reference)

Catalog No. ABIN731513

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100 μL

Shipping to: United States

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Quick Overview for ATP7A antibody (AA 242-285) (ABIN731513)

Target

See all ATP7A Antibodies

ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Reactivity

Human, Rat, Mouse

Host

Rabbit

Clonality

Polyclonal

Conjugate

This ATP7A antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product ATP7A Primary Antibody

Binding Specificity
14

13

6

5

2

1

1

1

1

1

1

1

1
AA 242-285

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Horse,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human ATP7A

Isotype

IgG
anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (HRP)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 HRP

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (FITC)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 FITC

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (Biotin)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 Biotin

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (PE)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 PE

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (APC)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 APC

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (PerCP)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 PerCP

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (Atto 488)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 Atto 488

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (Atto 594)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 Atto 594

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody (Atto 390)
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 Atto 390

anti-ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A) (AA 42-61) antibody
ATP7A Reactivity: Human WB, IHC, IP, IF, ICC Host: Mouse Monoclonal S60-4 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Wang, Zhu, Zhao, Wang: "miR-133a enhances the sensitivity of Hep-2 cells and vincristine-resistant Hep-2v cells to cisplatin by downregulating ATP7B expression." in: International journal of molecular medicine, Vol. 37, Issue 6, pp. 1636-42, (2016) (PubMed).
Target

ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Alternative Name

Atp7a

Background

Synonyms: MK, MNK, DSMAX, SMAX3, Copper-transporting ATPase 1, Copper pump 1, Menkes disease-associated protein, ATP7A, MC1
Background: May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.

Gene ID

538

UniProt

Q04656

Pathways

Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process