ACTN2 antibody (AA 557-692) (CF®647)

Catalog No. ABIN7649123

The Mouse Monoclonal anti-ACTN2 antibody has been validated for WB, IHC and IHC (fp). It is suitable to detect ACTN2 in samples from Human.

Quick Overview for ACTN2 antibody (AA 557-692) (CF®647) (ABIN7649123)

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTN2 antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ACTN2-3294

Product Details anti-ACTN2 Antibody (CF®647)

Binding Specificity: AA 557-692

Purpose: Sarcomeric Actinin Alpha 2 / ACTN2(ACTN2/3294), CF647 conjugate

Characteristics: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant fragment (aa557-692) of human ACTN2 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Skeletal or Cardiac Muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ACTN2

Target: ACTN2 (Actinin, alpha 2 (ACTN2))

Alternative Name: ACTN2

Background:

Synonyms: Actin binding protein, Alpha actinin skeletal muscle, isoform 2, CMD1AA, F-actin cross-linking protein

Gene Symbol: ACTN2

Tissue Expression: Muscle

Molecular Weight: 103 kDa

Gene ID: 88

UniProt: P35609

Pathways: Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity