APC antibody (AA 1-433) (CF®405S)

Catalog No. ABIN7649170

This Mouse Monoclonal antibody specifically detects APC in WB, IHC, IP and IF. It exhibits reactivity toward Human.

Quick Overview for APC antibody (AA 1-433) (CF®405S) (ABIN7649170)

Target: APC (Adenomatous Polyposis Coli (APC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APC antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: ALi 12-28

Product Details anti-APC Antibody (CF®405S)

Binding Specificity: AA 1-433

Purpose: APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor)(ALi 12-28), CF405S conjugate

Characteristics: Adenomatous Polyposis Coli (APC) is a protein with many cellular functions. It is a tumor suppressor and negative regulator of Wnt signaling. APC has roles in cell adhesion and cell migration. Mutations in APC are assuciated with several diseases. The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human APC protein (around aa 1-433)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: Adenomatous Polyposis Coli

Background:

Synonyms: Adenomatous Polyposis Coli, APC, CC1, Deleted in polyposis 2.5, Protein Phosphatase 1, Regulatory Subunit 46, DP2, DP3

Gene Symbol: APC

Molecular Weight: 66 kDa

Gene ID: 324

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization