ATG5 antibody (AA 1-119) (CF®647)

Catalog No. ABIN7649695

This Mouse Monoclonal antibody specifically detects ATG5 in WB, IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for ATG5 antibody (AA 1-119) (CF®647) (ABIN7649695)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG5 antibody is conjugated to CF®647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ATG5-2492

Product Details anti-ATG5 Antibody (CF®647)

Binding Specificity: AA 1-119

Purpose: ATG5 (Autophagy Marker)(ATG5/2492), CF647 conjugate

Characteristics: Autophagy protein 5 (ATG5), in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. ATG5 is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy, a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human ATG5 protein (around aa 1-119)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: THP1, PANC-1, Raji or HeLa cells. Brain, Ovary, Endometrium, Colon or Duodenum (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background:

Synonyms: Autophagy protein 5, ATG5, Autophagy related 5, APG5-like, Apoptosis-specific protein

Gene Symbol: ATG5

Molecular Weight: 50-32 kDa

Gene ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy