Aurora Kinase B antibody (AA 89-251) (CF®647)

Catalog No. ABIN7649750

The Mouse Monoclonal anti-Aurora Kinase B antibody has been validated for IHC and IHC (fp). It is suitable to detect Aurora Kinase B in samples from Human.

Quick Overview for Aurora Kinase B antibody (AA 89-251) (CF®647) (ABIN7649750)

Target: Aurora Kinase B (AURKB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora Kinase B antibody is conjugated to CF®647

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: AURKB-1845

Product Details anti-Aurora Kinase B Antibody (CF®647)

Binding Specificity: AA 89-251

Purpose: Aurora B (Proliferation Marker)(AURKB/1845), CF647 conjugate

Characteristics: This antibody recognizes a protein of 39 kDa, which is identified as Aurora kinase B (or Aurora B). Aurora B is a serine/threonine protein kinase and component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. It is important for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 89-251) of human Aurora B protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa cells. Tonsil, Placenta, Hodgkin Lymphoma or Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: Aurora kinase B

Background:

Synonyms: Aurora kinase B, Aurora B, Aurora-1, AURKB, AIK2, AIM-1, ARK-2, AurB, Aurora and Ipl1 like midbody associated protein 1, Aurora-B, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, IPL1, Protein phosphatase 1 regulatory subunit 48, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase aurora-B, STK1, STK12, STK5

Gene Symbol: AURKB

Molecular Weight: 39 kDa

Gene ID: 9212

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades