Recombinant BCL10 antibody (AA 122-168) (CF®405S)

Catalog No. ABIN7649839

This Rabbit Monoclonal antibody specifically detects BCL10 in IHC, IF, FACS and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Recombinant BCL10 antibody (AA 122-168) (CF®405S) (ABIN7649839)

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BCL10 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: BL10-2988R

Product Details anti-BCL10 Antibody (CF®405S)

Binding Specificity: AA 122-168

Purpose: BCL10 (MALT-Lymphoma Marker) (BL10/2988R), CF405S conjugate

Characteristics: B-cell lymphoma/leukemia 10 (BCL10) is a signaling protein that promotes apoptosis, pro-caspase-9 maturation, and activation of NF-kappa-B via NIK and IKK. BCL10 contains an N-terminal caspase recruitment domain (CARD), which is found in a number of apoptotic regulatory molecules. BCL10 is involved in the adaptive immune response, and may be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. A BCL10 translocation is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BCL10 protein fragment (around aa122-168)

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HepG2 cells or Lymphoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for BCL10

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Alternative Name: BCL10

Background:

Synonyms: BCL10, BCL-10, B-cell CLL/lymphoma 10, B-cell leukemia/lymphoma 10

Gene Symbol: BCL10

Molecular Weight: 33 kDa

Gene ID: 8915

UniProt: O95999

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins