BCL2L1 antibody (AA 1-135) (CF®640R)

Catalog No. ABIN7650006

This anti-BCL2L1 antibody is a Mouse Monoclonal antibody detecting BCL2L1 in . Suitable for Human.

Quick Overview for BCL2L1 antibody (AA 1-135) (CF®640R) (ABIN7650006)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL2L1 antibody is conjugated to CF®640R

Application: Please inquire

Clone: BCL2L1-1762

Product Details anti-BCL2L1 Antibody (CF®640R)

Binding Specificity: AA 1-135

Purpose: Bcl-X (Apoptosis Marker)(BCL2L1/1762), CF640R conjugate

Characteristics: Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BCL2L1 protein fragment (around aa 1-135)

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: Bcl-x

Background:

Synonyms: Bcl-x, Bcl-2-like protein 1, BCL2L1, Apoptosis regulator Bcl-X

Gene Symbol: BCL2L1

Molecular Weight: 27 kDa

Gene ID: 598

UniProt: Q07817

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling