Recombinant Cadherin-16 antibody (CF®740)

Catalog No. ABIN7650259

This Mouse Monoclonal antibody specifically detects Cadherin-16 in IHC, WB, FACS and IHC (fp). It exhibits reactivity toward Human, Mouse, Rat, Dog and Rabbit.

Quick Overview for Recombinant Cadherin-16 antibody (CF®740) (ABIN7650259)

Target: Cadherin-16 (CDH16)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RCDH16-1071

Product Details anti-Cadherin-16 Antibody (CF®740)

Purpose: Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 (rCDH16/1071), CF740 conjugate

Characteristics: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CDH16 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA Buffer pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Normal kidney or renal cell carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: CDH16

Background:

Synonyms: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody

Gene Symbol: CDH16

Tissue Expression: Epithelial cells

Molecular Weight: 130 kDa

Gene ID: 1014

UniProt: O75309