Calcineurin A antibody (CF®405S)

Catalog No. ABIN7650358

This Mouse Monoclonal antibody specifically detects Calcineurin A in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for Calcineurin A antibody (CF®405S) (ABIN7650358)

Target: Calcineurin A (CAN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calcineurin A antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: CALNA-2353

Product Details anti-Calcineurin A Antibody (CF®405S)

Purpose: Calcineurin A / PPP3CA(CALNA/2353), CF405S conjugate

Characteristics: This antibody recognizes the Calcineurin A alpha protein, encoded by the PPP3CA (Protein Phosphatase 3 Catalytic Subunit Alpha) gene. Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Calcineurin A protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Calcineurin A

Target: Calcineurin A (CAN)

Alternative Name: Calcineurin A

Background:

Synonyms: Calcineurin A, Calcineurin A alpha, PPP3CA, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CALN, CALNA1, CAM-PRP catalytic subunit, CCN1, CNA alpha, CNA1, Protein phosphatase 3 catalytic subunit alpha isoform

Tissue Expression: Brain|T-cells

Molecular Weight: 62 kDa

Gene ID: 5530

UniProt: Q08209