Caldesmon antibody (High Molecular Weight) (CF®568)

Catalog No. ABIN7650403

This Rabbit Polyclonal antibody specifically detects Caldesmon in WB, IHC and IHC (fp). It exhibits reactivity toward Human and Rat.

Quick Overview for Caldesmon antibody (High Molecular Weight) (CF®568) (ABIN7650403)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caldesmon antibody is conjugated to CF®568

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-Caldesmon Antibody (CF®568)

Binding Specificity: High Molecular Weight

Purpose: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker), CF568 conjugate

Characteristics: Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CALD1 protein fragment

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: Caldesmon

Background:

Synonyms: Caldesmon, Caldesmon 1, Caldesmon 1 Isoform 1, CDM, HCAD

Gene Symbol: CALD1

Tissue Expression: Smooth muscle

Molecular Weight: 150 kDa

Gene ID: 800

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction