Recombinant Caldesmon antibody (High Molecular Weight) (Biotin)

Catalog No. ABIN7650416

The Mouse Monoclonal anti-Caldesmon antibody has been validated for WB, IHC and IHC (fp). It is suitable to detect Caldesmon in samples from Human.

Quick Overview for Recombinant Caldesmon antibody (High Molecular Weight) (Biotin) (ABIN7650416)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RCALD1-820

Product Details anti-Caldesmon Antibody (Biotin)

Binding Specificity: High Molecular Weight

Purpose: Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (rCALD1/820), Biotin conjugate

Characteristics: Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb This antibody recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CALD1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat. Immunohistology (formalin): 0.25-0.5 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA pH 7.5-8.5 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: Caldesmon

Background:

Synonyms: Caldesmon, Caldesmon 1, Caldesmon 1 Isoform 1, CDM, HCAD

Gene Symbol: CALD1

Tissue Expression: Smooth muscle

Molecular Weight: 150 kDa

Gene ID: 800

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction